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Key Documents

MAB333

Sigma-Aldrich

Anti-Synaptobrevin Antibody, clone SP10

ascites fluid, clone SP10, Chemicon®

Sinónimos:

VAMP

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

SP10, monoclonal

reactividad de especies

rat, hamster, salamander, pig, human

fabricante / nombre comercial

Chemicon®

técnicas

immunohistochemistry: suitable
western blot: suitable

isotipo

IgM

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... VAMP1(6843)

Especificidad

Synaptobrevin [VAMP]. MAB333 reacts with VAMP-1 and VAMP-2 fusion protein expressed in bacterial system.

Inmunógeno

Crude synaptic immunoprecipitate (Human).

Aplicación

Anti-Synaptobrevin Antibody, clone SP10 is an antibody against Synaptobrevin for use in WB, IH.
Immunohistochemistry at >1:25.

Western blotting at 1:100-1:10,000.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Ligadura / enlace

Replaces: AB5863P

Forma física

Liquid, contains sodium azide.

Almacenamiento y estabilidad

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids


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S E Bradford et al.
Neuroscience, 128(4), 751-765 (2004-10-07)
Certain excitatory pathways in the rat hippocampus can release aspartate along with glutamate. This study utilized rat hippocampal synaptosomes to characterize the mechanism of aspartate release and to compare it with glutamate release. Releases of aspartate and glutamate from the
G Bonanno et al.
British journal of pharmacology, 129(8), 1780-1786 (2000-04-26)
The release of [(3)H]-dopamine ([(3)H]-DA) from human neocortex nerve terminals was studied in synaptosomes prepared from brain specimens removed in neurosurgery and exposed during superfusion to different releasing stimuli. Treatment with 15 mM KCl, 100 microM 4-aminopyridine, 1 microM ionomycin
D M Sherry et al.
The Journal of comparative neurology, 431(4), 424-436 (2001-02-27)
Vesicle associated membrane protein (VAMP; also known as synaptobrevin) is a key component of the core complex needed for docking and fusion of synaptic vesicles with the presynaptic plasma membrane. Recent work indicates that the precise complement of presynaptic proteins
Peter Nagele et al.
Anesthesiology, 103(4), 768-778 (2005-09-30)
Volatile general anesthetics (VAs) have a number of synaptic actions, one of which is to inhibit excitatory neurotransmitter release; however, no presynaptic VA binding proteins have been identified. Genetic data in Caenorhabditis elegans have led to the hypothesis that a
S E Ahmari et al.
Nature neuroscience, 3(5), 445-451 (2000-04-19)
Little is known about presynaptic assembly during central nervous system synaptogenesis. Here we used time-lapse fluorescence imaging, immunocytochemistry and electron microscopy to study hippocampal neuronal cultures transfected with a fusion construct of the presynaptic vesicle protein VAMP and green fluorescent

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