MAB3222
Anti-BrdU Antibody, clone IIB5
clone IIB5, Chemicon®, from mouse
Sinónimos:
BrdU
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
IIB5, monoclonal
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Chemicon®
técnicas
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
isotipo
IgG1
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Categorías relacionadas
Descripción general
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Especificidad
Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
Inmunógeno
A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
Aplicación
Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Forma física
Format: Purified
Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.
Protein A purified
Almacenamiento y estabilidad
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análisis
Control
After incorporation of BrdU, all DNA containing species
After incorporation of BrdU, all DNA containing species
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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