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Key Documents

MAB316

Sigma-Aldrich

Anti-GABA Antibody, clone 5A9

clone 5A9, Chemicon®, from mouse

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

5A9, monoclonal

reactividad de especies (predicha por homología)

mammals

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG1

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... GABRA1(2554)

Especificidad

Specific for GABA and does not cross-react with other amino acids.

Inmunógeno

GABA-coupled to BSA with glutaraldehyde.

Aplicación

Anti-GABA Antibody, clone 5A9 detects level of GABA & has been published & validated for use in ELISA, IH & WB.
Immunohistochemistry on frozen sections if perfusion fixed. Glutaraldehyde must be included in fixative with paraffin sections: 1:200-1:400. Typical fixative might be 4% PFA, 1% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.3 .

Immunoblotting not recommended due to low molecular wgt: 103Da.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Forma física

Format: Purified
Protein A purified
Purified mouse monoclonal in PBS, pH 7.4, 1% BSA, and 0.1% sodium azide as a preservative.

Almacenamiento y estabilidad

Maintain refrigerated at 2–8°C for up to 6 months.

Nota de análisis

Control
Myeloblasts, lymphocytes, erythroblasts

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Alain C Burette et al.
The Journal of comparative neurology, 522(9), 2164-2178 (2014-03-19)
The 53 kDa insulin receptor substrate protein (IRSp53) is highly enriched in the brain. Despite evidence that links mutations of IRSp53 with autism and other neuropsychiatric problems, the functional significance of this protein remains unclear. We used light and electron
GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat Dentate Gyrus.
Halasy, K., et al.
The European Journal of Neuroscience, 4, 144-153 (1992)
E Szabat et al.
Neuroscience, 47(2), 409-420 (1992-01-01)
A monoclonal antibody, 115AD5, was raised against GABA coupled to bovine serum albumin. The monoclonal antibody 115AD5 also reacted with other GABA-protein conjugates. The specificity of the monoclonal antibody was corroborated by enzyme-linked immunoassay, dot-immunobinding experiments and immunostaining of rat
Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis.
Oscar A Bizzozero,Jianzheng Zheng
Journal of Neuroscience Research null
S Westerholz et al.
Neuroscience, 168(2), 573-589 (2010-03-27)
Early in development spontaneous activity modulates survival and connectivity of neurons and thus plays a crucial role in the formation of neural networks. The emergence of synchronous activity in cultured neocortical networks initially is driven by large GABAergic interneurons. Here

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