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MAB3118

Sigma-Aldrich

Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11

ascites fluid, clone 2EL-1B11, Chemicon®

Sinónimos:

Anti-FLAG Antibody, FLAG Tag Antibody

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

2EL-1B11, monoclonal

reactividad de especies

vertebrates, E. coli

reactividad de especies (predicha por homología)

rat

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG1

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Descripción general

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Especificidad

DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.

Inmunógeno

Epitope: Epitope tag
Synthetic peptide M-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys(C) coupled to ovalbumin

Aplicación

Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.
Immunofluorescence:
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.

Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.

ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.

Immunoblotting:
on recombinant proteins

Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Calidad

Routinely evaluated by Western Blot on Flag-mTor protein blot.

Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.

Ligadura / enlace

Replaces: AB3250

Forma física

Ascites mouse monoclonal IgG1 liquid containing no preservative.
Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Cells transfected with FLAG-tagged fusion vector

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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