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Documentos clave

MAB3101

Sigma-Aldrich

Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 5B9.2

clone 5B9.2, Chemicon®, from mouse

Sinónimos:

Cx45

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

5B9.2, monoclonal

reactividad de especies

rat, rabbit, human

fabricante / nombre comercial

Chemicon®

técnicas

immunocytochemistry: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... GJC1(10052)
rat ... Gjc1(266706)

Especificidad

Recognizes Human Connexin 45.

SPECIES REACTIVITIES:

Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.

Inmunógeno

Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.

Aplicación

Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 5B9.2 detects level of Connexin 45 & has been published & validated for use in IC & WB.
Immunblotting (~46 kDa)

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Forma física

Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25 M NaCl, pH=7.6 containing 0.1% sodium azide.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina.
Alexandre Hiroaki Kihara, Leandro Mantovani de Castro, Monica Aparecida Belmonte et al.
Journal of Neurobiology null
Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1.
Li, X; Kamasawa, N; Ciolofan, C; Olson, CO; Lu, S; Davidson, KG; Yasumura, T; Shigemoto et al.
The Journal of Neuroscience null
Axonal synapses utilize multiple synaptic ribbons in the mammalian retina.
Kim, HL; Jeon, JH; Koo, TH; Lee, UY; Jeong, E; Chun, MH; Moon, JI; Massey, SC; Kim, IB
Testing null
Electrical synapses in retinal ON cone bipolar cells: subtype-specific expression of connexins.
Han, Y; Massey, SC
Proceedings of the National Academy of Sciences of the USA null
Alexandre H Kihara et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 28(1), 39-52 (2009-10-06)
Connexin (Cx) channels and hemichannels are involved in essential processes during nervous system development such as apoptosis, propagation of spontaneous activity and interkinetic nuclear movement. In the first part of this study, we extensively characterized Cx gene and protein expression

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