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Key Documents

MAB3075

Sigma-Aldrich

Anti-G Protein Giα-1 Antibody, clone R4

ascites fluid, clone R4, Chemicon®

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

R4, monoclonal

reactividad de especies

guinea pig, bovine, mouse, rat, human

fabricante / nombre comercial

Chemicon®

técnicas

western blot: suitable

isotipo

IgG2b

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

bovine ... Gnai1(281790)
human ... GNAI1(2770)
mouse ... Gnai1(14677)
rat ... Gnai1(25686)

Especificidad

Gialpha-1. Strong reactivity with rat (100%), mouse (78%), bovine (74%) and guinea pig (48%). Slight reactivity with human (31%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.

Inmunógeno

Rat brain G-proteins purified by the method of Sternweis and Robishaw (Sternweis & Robishaw, 1984).

Aplicación

Anti-G Protein Giα-1 Antibody, clone R4 detects level of G Protein Giα-1 & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:500 - 1:2000.

Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Optimal working dilutions must be determined by end user.

Forma física

Liquid ascites, containing sodium azide as a preservative.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.
Sternweis, P C and Robishaw, J D
The Journal of Biological Chemistry, 259, 13806-13813 (1984)
E Burgos-Ramos et al.
Neuroscience, 154(4), 1458-1466 (2008-06-17)
Minocycline is a semi-synthetic second-generation tetracycline known to improve cognition in amyloid precursor protein transgenic mice. Whether it can protect the somatostatin (SRIF) receptor-effector system, also involved in learning and memory, from alterations induced by chronic i.c.v. infusion of beta-amyloid
Activity of the hippocampal somatostatinergic system following daily administration of melatonin.
Rosa Maria Izquierdo-Claros, Maria Del Carmen Boyano-Adanez, Eduardo Arilla-Ferreiro
Brain Research Molecular Brain Research null
Acutely administered melatonin decreases somatostatin-binding sites and the inhibitory effect of somatostatin on adenylyl cyclase activity in the rat hippocampus.
Rosa Maria Izquierdo-Claros, Maria del Carmen Boyano-Adanez Md, Eduardo Arilla-Ferreiro
Journal of Pineal Research null
Apurva A Javkhedkar et al.
Physiological reports, 3(11) (2015-11-26)
Previously we have shown that in spontaneously hypertensive rats (SHR) renal angiotensin (Ang) II receptor (AT1R) upregulation leads to overstimulation of Na/K-ATPase by Ang II. There are reports that antioxidants can reduce oxidative stress and blood pressure (BP) in SHR

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