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MAB2137

Sigma-Aldrich

Anti-Homing Receptor Antibody, clone A020

clone A020, Chemicon®, from rat

Sinónimos:

CD44s, HCAM, Pgp-1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rat

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

A020, monoclonal

reactividad de especies

mouse, human, rat, pig, rabbit, canine, bovine

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG2b

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CD44(960)

Especificidad

Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).

Inmunógeno

Purified human blood lymphocyte CD44

Aplicación

Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.
ELISA 1:2000

Flow Cytometry :1:1000

Immunofluorescence 1:200

Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

Immunoprecipitation 1:200

Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Descripción de destino

90 kDa

Forma física

Format: Purified
Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.
Protein A purified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Cells of epithelium and highly expressed by carcinomas

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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M Z Gilcrease et al.
Cancer, 86(11), 2320-2326 (1999-12-11)
Recent reports have shown altered expression of CD44 in renal cell carcinomas. However, to the authors' knowledge there are no data correlating CD44 expression in renal cell carcinomas with subsequent tumor progression or recurrence, nor is there information about the
Milan Makwana et al.
The Journal of comparative neurology, 518(5), 699-721 (2009-12-25)
Generation of new axonal sprouts plays an important role in neural repair. In the current study, we examined the appearance, composition and effects of gene deletions on intrabrainstem sprouts following peripheral facial nerve axotomy. Axotomy was followed by the appearance
Mature human thymocytes migrate on laminin-5 with activation of metalloproteinase-14 and cleavage of CD44.
Vivinus-Nebot, Mylene, et al.
Journal of immunology (Baltimore, Md. : 1950), 172, 1397-1406 (2004)
Sameerah Shaheen et al.
Stem cell reviews and reports, 12(4), 492-499 (2016-05-22)
Colorectal cancers (CRCs) form a disorganized hierarchy of heterogeneous cell populations on which current chemotherapy regimens fail to exert their distinctive cytotoxicity. A small sub-population of poorly differentiated cancer stem-like cells (CSCs), also known as cancer initiating cells, may exhibit
Carmela Ricciardelli et al.
BMC cancer, 13, 476-476 (2013-10-16)
Hyaluronan (HA) an important component of the extracellular matrix, has been linked to tumor progression and drug resistance in several malignancies. However, limited data is available for ovarian cancer. This study investigated the role of hyaluronan (HA) and a potential

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