MAB1951Z
Anti-Integrin β1 Antibody, clone P4G11, azide free
clone P4G11, Chemicon®, from mouse
Sinónimos:
CD29, MAB1951
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
P4G11, monoclonal
reactividad de especies
monkey, human, rabbit
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ITGB1(3688)
Especificidad
Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.
Inmunógeno
Human IL-1beta activated endothelial cells
Aplicación
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.
Forma física
Format: Purified
Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.
Almacenamiento y estabilidad
Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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