MAB16985

Anti-MCAM Antibody, clone P1H12

clone P1H12, Chemicon^®, from mouse

• Sinónimos: CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: P1H12, monoclonal
• application: ELISA; FACS; ICC; IHC; IP; WB
• reactividad de especies: mouse, canine, human
• técnicas: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 20

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: P1H12, monoclonal
• reactividad de especies: mouse, canine, human
• no debe reaccionar con: rat
• envase: antibody small pack of 25 μg
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1
• idoneidad: not suitable for immunohistochemistry (Paraffin)
• Nº de acceso NCBI: NM_006500.2
• Nº de acceso UniProt: P43121
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MCAM(4162)

Descripción

Descripción general

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

Especificidad

Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.

Inmunógeno

Immunization of mice with HUVEC.

Aplicación

Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.

Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.

Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.

ELISA:
1-10 μg/mL of a previous lot worked in ELISA.

FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.

Optimal working dilutions must be determined by end user.

Calidad

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.

Descripción de destino

110 kDa

Forma física

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.

Nota de análisis

Control
HUVEC cells.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable