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Key Documents

MAB1281A4

Sigma-Aldrich

Anti-Nuclei Antibody, clone 235-1, Alexa Fluor 488 conjugate

clone 235-1, from mouse, ALEXA FLUOR 488

Sinónimos:

HuNu

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

ALEXA FLUOR 488

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

235-1, monoclonal

reactividad de especies

human

técnicas

immunocytochemistry: suitable

isotipo

IgG1

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.

Inmunógeno

Human nuclei

Aplicación

Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in H9 human embryonic stem cells. Evaluated by Immunocytochemistry in mouse embryonic fibroblasts. Immunocytochemistry Analysis: A 1:50 dilution of this antibody did not detect nuclei in mouse embryonic fibroblasts.
Research Category
Stem Cell Research

Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Developmental Neuroscience

Mesenchymal Stem Cells

Neurodegenerative Diseases
The anti-Nuclei, clone 235-1, Alexa Fluor 488 conjugate will recognize human cell nuclei only & is usefull in xenograph transplantation studies to study stem cell migration.

Calidad

Evaluated by Immunocytochemistry in human foreskin fibroblasts (SCC058). Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected nuclei in human foreskin fibroblasts (SCC058).

Forma física

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Nota de análisis

Control
Human foreskin fibroblasts (SCC058)

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sacha M L Khong et al.
Stem cells (Dayton, Ohio), 37(2), 240-246 (2018-11-10)
Although bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely recognized as promising therapeutic agents, the age-related impacts on cellular function remain largely uncharacterized. In this study, we found that BM-MSCs from young donors healed wounds in a xenograft model faster
Nadezhda A Evtushenko et al.
International journal of molecular sciences, 22(8) (2021-05-01)
The recessive form of dystrophic epidermolysis bullosa (RDEB) is a crippling disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Using ectopic expression of hTERT/hTERT + BMI-1 in primary cells, we developed

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