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Key Documents

MAB1202

Sigma-Aldrich

Anti-CD42a Antibody, platelet lb-IX, clone FMC-25

clone FMC-25, Chemicon®, from mouse

Sinónimos:

Anti-CD42a, Anti-GPIX

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

FMC-25, monoclonal

reactividad de especies

human

fabricante / nombre comercial

Chemicon®

técnicas

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... GP9(2815)

Especificidad

This antibody belongs to CD42a (assigned at the Fourth International Workshop on Leukocyte Differentiation Antigens, Vienna, 1989) (Knapp, 1989), and binds to the 22,000 dalton IX subunit of the glycoprotein Ib-IX complex (Zola et al., 1984; Berndt et al., 1985a,b). FMC-25 failed to bind to platelets from patients with Bernard-Soulier Syndrome (Berndt et al., 1983). The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype (San Miguel et al., 1985, 1986).

Cell reactivity:

The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype(San Miguel et al., 1985, 1986).

Inmunógeno

Epitope: platelet lbalpha complex
Peripheral blood mononuclear cells isolated on Ficoll® hypaque.

Aplicación

Anti-CD42a Antibody, platelet lb-IX, clone FMC-25 detects level of CD42a & has been published & validated for use in FC, IC, IH.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
The glycoprotein Ib-IX complex on the surface of normal platelets is a heterotrimer of Ib-alpha, Ib-beta and IX subunits (Berndt, 1989). Ib-IX complex is an essential component of platelet adhesion to vascular subendothelium and is also important in the maintenance of platelet shape (Berndt et al., 1988).

This antibody is suitable for enumeration of platelets by flow cytometry and immunoperoxidase staining of frozen tissue sections or air dried smear preparations (San Miguel et al., 1986), and for identification of megakaryoblasts in acute myeloid leukemia or acute myelofibrosis (San Miguel et al., 1985; San Miguel et al., 1986).

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:25

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 platelets in 100 μl of buffer.

2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:20 will be applicable to most commonly used systems.

Forma física

Format: Purified
Purified immunoglobulin. The antibody is supplied in 25mM Tris-HCl, 0.4M NaCl, pH 8.0 containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Almacenamiento y estabilidad

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Ficoll is a registered trademark of Cytiva

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8B - Non-combustible corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

N Rosenberg et al.
Journal of thrombosis and haemostasis : JTH, 5(2), 378-386 (2006-11-07)
Bernard-Soulier syndrome (BSS) is a severe inherited bleeding disorder that is caused by a defect in glycoprotein (GP)Ib-IX-V complex, the platelet membrane receptor for von Willebrand factor. The diagnosis of BSS was made in two members of a Bukharian Jewish

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