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Sigma-Aldrich

Anti-Cathepsin D (Ab-2) Rabbit pAb

liquid, Calbiochem®

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About This Item

Código UNSPSC:
12352203

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

liquid

contiene

≤0.1% sodium azide as preservative

reactividad de especies

human

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

do not freeze

isotipo

IgG

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CTSD(1509)

Descripción general

Anti-Cathepsin D (Ab-2), rabbit polyclonal, recognizes the ~52 kDa latent form and ~34 kDa subunit in breast carcinoma tissue. It is validated for WB and IHC with frozen and paraffin sections.
Purified rabbit polyclonal antibody. Recognizes the ~52 kDa latent form and the ~34 kDa (but not the ~14 kDa) active subunit of cathepsin D.
Recognizes the ~52 kDa latent form D and the ~34 kDa subunit, but not the ~14 kDa subunit of cathepsin D in breast carcinoma tissue extracts.

Inmunógeno

Human
purified human liver cathepsin D

Aplicación

Frozen Sections (5 µg/ml)

Immunoblotting (2.5 µg/ml)

Paraffin Sections (5 µg/ml, trypsin pre-treatment required)

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Nota de análisis

Positive Control
HT1080 concentrated conditioned medium or breast carcinoma tissue

Otras notas

For optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
Rochefort, H. 1992. Eur. J. Cancer28A, 1780.
Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell20, 352.
Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10-13 - German Storage Class 10 to 13


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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