HTS101M

CHEMISCREEN MEMBRANE PREPARATION RECOMBINANT HUMAN µ (Mu) OPIOID RECEPTOR

Human Mu / OP3 / MOP / MOR GPCR membrane preparation for GTPγS Binding & Radioligand Binding Assays.

• Sinónimos: ChemiSCREEN Receptor Kit, µ-Opioid Receptor Membrane Prep
• Código UNSPSC: 41106514
• eCl@ss: 32161000
• NACRES: NA.41

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in Chem-5 cells
• fabricante / nombre comercial: ChemiScreen; Chemicon^®
• técnicas: ligand binding assay: suitable (GTPγS); radioligand binding assay (RLBA): suitable
• Nº de acceso NCBI: NM_000914.2; NM_001008503.1; NM_001008504.1; NM_001008505.1
• Nº de acceso UniProt: P35372
• Condiciones de envío: dry ice
• Información sobre el gen: human ... OPRM1(4988)

Descripción

Descripción general

Full-length human OPRM1 cDNA encoding Mu

Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, µ κ and δ that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the µ opioid receptor (Dhawan et al., 1996). Chemicon′s µ opioid receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of µ opioid receptor interactions with its ligands. The membrane preparations exhibit an EC50 of 1.6nM for DAMGO in a GTPγS binding assay.

Aplicación

GTPγS Binding and Radioligand Binding Assay.

Acciones bioquímicas o fisiológicas

GPCR Class: A

Protein Target: Mu / OP3 / MOP / MOR

Target Sub-Family: Opioid

Calidad

EC50 in GTPγS binding assay by DAMGO: ~ 1.6 nM

Especificaciones

Inucbation Conditions
ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [³⁵S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl₂/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled DAMGO added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water, and washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4. The plate is dried and counted.

One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific DAMGO-stimulated [³⁵S]-GTPγS binding.
The μ opioid receptor membrane preparation is expected to be functional in a radioligand binding assay; however, the end user will need to determine the optimal radiolabeled ligand for use with this product.

Forma física

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C

Almacenamiento y estabilidad

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2