HTS100RTA
Ready-to-Assay delta Opioid Receptor Frozen Cells
Human
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About This Item
Código UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81
Productos recomendados
product name
Ready-to-Assay delta Opioid Receptor Frozen Cells, Human Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.
origen biológico
human
Nivel de calidad
fabricante / nombre comercial
Ready-to-Assay
técnicas
calcium flux assay: suitable
Nº de acceso NCBI
método de detección
fluorometric
Condiciones de envío
dry ice
Descripción general
Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.
Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.
Descripción línea celular
GPCR Cell Lines
Host cells: Chem-1
Acciones bioquímicas o fisiológicas
GPCR Class: A
Protein Target: Delta / OP1 / DOP / DOR
Target Sub-Family: Opioid
Componentes
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Otras notas
Limited to a single use and shall not be propagated and/or re-frozen by licensee.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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