GR15L

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

lyophilized, clone EGFR.1, Calbiochem^®

• Sinónimos: Anti-Epidermal Growth Factor Receptor
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: EGFR.1, monoclonal
• formulario: lyophilized
• no contiene: preservative
• reactividad de especies: human
• no debe reaccionar con: mouse, rat
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• isotipo: IgG2b
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Descripción

Descripción general

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.

Recognizes the ~170 kDa EGF receptor protein in A431 cells.

Inmunógeno

Human

partially purified, human EGFR from A431 cells

Aplicación

Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; see application references)

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitución

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Nota de análisis

Negative Control
HL-60 cells

Positive Control
A-431 cells

Otras notas

Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1