GR13L

Anti-EGFR (Ab-3) Mouse mAb (225)

lyophilized, clone 225, Calbiochem^®

• Sinónimos: Anti-EGF-R
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 225, monoclonal
• formulario: lyophilized
• no contiene: preservative
• reactividad de especies: human
• no debe reaccionar con: rat, mouse
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• isotipo: IgG1
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Descripción

Descripción general

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

Inmunógeno

Human

partially purified, human EGFR from A431 cells

Aplicación

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

Reconstitución

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Nota de análisis

Negative Control
HL-60 cells

Positive Control
A431 cells

Otras notas

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable