FCMAB191F

Milli-Mark^® Anti-CD27-FITC Antibody, clone M-T271

clone M-T271, Milli-Mark^®, from mouse

• Sinónimos: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.44
• conjugado: FITC conjugate
• application: FACS
• clon: M-T271, monoclonal
• reactividad de especies: human
• técnicas: flow cytometry: suitable

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: FITC conjugate
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: M-T271, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Milli-Mark^®
• técnicas: flow cytometry: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001233.1
• Nº de acceso UniProt: P26842
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CD27(939)

Descripción

Descripción general

CD27, a member of the nerve growth factor receptor (NGFR) superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kDa homodimer (Bigler, 1988; Sugita, 1992; Morimoto, 1995). CD27 is expressed on mature thymocytes and on the majority of human peripheral blood T cells, on both CD4+ and CD8+ subsets. CD27 is also expressed on activated B cells and a fraction of resting natural killer (NK) cells (Maurer, 1990; Sugita, 1992). Among CD4+ T cells, CD27 is preferentially expressed on unprimed CD4+CD45RA+CD45R0- T cells, while primed CD4+CD45RA-CD45R0+ T cells express low levels of CD27 (Sugita, 1992). During activation, the expression of CD27 is increased on B cells and unprimed T cells, while primed T cells have a relatively low capacity for upregulation of CD27 (Morimoto, 1995; Hintzen, 1994). The activation is also accompanied by the appearance of a 32 kDa soluble form of CD27 (sCD27) found in supernatants of activated lymphocytes (Hintzen, 1994).
CD27 is believed to play a role in T cell activation, since the expression is upregulated during T cell activation (Bigler, 1988; Sugita, 1992; Morimoto, 1995; Maurer, 1990; Hintzen, 1994; Lens, 1995).

Especificidad

Antibody recognizes Human CD27 antigen.

Inmunógeno

Leukemic peripheral blood T cells from a patient with chronic T cell leukemia (T-CLL)

Aplicación

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.

Calidad

Evaluated by flow cytometry using human PBMCs

Descripción de destino

120 kDa (Bigler, 1988; Sugita, 1992; Morimoto, 1995)

Forma física

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Almacenamiento y estabilidad

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Nota de análisis

Control
PBMCs

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable