FCMAB116P

Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70

clone MC-813-70, Milli-Mark^®, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: PE
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: MC-813-70, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Milli-Mark^®
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable
• isotipo: IgG3κ
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FUT4(2526)

Descripción

Descripción general

N-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.

The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

Especificidad

This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Inmunógeno

Human embryonal carcinoma cell line 2102Ep.

Aplicación

Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugate (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.

Forma física

Protein A purified

Purified mouse monoclonal IgG3 conjugated to PE fluorochrome in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Nota de análisis

Control
Pluripotent human embryonic stem (ES) cells

Información legal

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable