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Key Documents

EZBRAIN42

Sigma-Aldrich

Human Amyloid β42 ELISA Kit

measures and quantifies Amyloid β42 levels in 50 μL tissue or cell lysates

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000

product name

Human Amyloid β42 Brain ELISA, This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.

Nivel de calidad

reactividad de especies

human

envase

kit of 1 × 96 wells

Parámetros

50 μL sample volume (Overnight assay)

assay range

sensitivity: 8 pg/mL
(lowest level of Amyloid β1-42 standard; 50 μL sample size)

standard curve range: 16-500 pg/mL

técnicas

ELISA: suitable

entrada

sample type tissue/cellular lysate

aplicaciones

research use

método de detección

colorimetric (450nm/590nm)

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Información sobre el gen

human ... APP(351)

Descripción general

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

Especificidad

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Aplicación

Research Category
Neuroscience
Research Sub Category
Alzheimer′s Disease
This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
This assay requires 50 µl of sample and is an overnight assay.
Used to detect/quantify: Amyloid β42

Almacenamiento y estabilidad

Components in the kit can be stored up to 2 weeks at 2-8°C

Otras notas

Please contact Technical Service for linearity of dilution.

Cláusula de descargo de responsabilidad

For research use only. Not for use in diagnostic procedures.

Pictogramas

CorrosionExclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Código de clase de almacenamiento

8A - Combustible, corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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