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DP02

Sigma-Aldrich

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

liquid, clone PAb416, Calbiochem®

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

PAb416, monoclonal

Formulario

liquid

contiene

≤0.1% sodium azide as preservative

reactividad de especies

SV40-infected cells

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

do not freeze

isotipo

IgG2a

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).

Inmunógeno

Epitope: Within amino acids 83-128
purified SV40 large T-antigen

Aplicación

Affinity Purification (use Cat. No. DP02A)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)

Paraffin Sections (see application references)

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Nota de análisis

Negative Control
3T3 cells
Positive Control
SV80 or SV-T2 cells

Otras notas

DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379.
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10, 591.
The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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