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Key Documents

CP01

Sigma-Aldrich

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem®

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

JLA20, monoclonal

formulario

liquid

contiene

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

reactividad de especies (predicha por homología)

all

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

isotipo

IgM

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ACTB(60)

Descripción general

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876
  • Inmunógeno

    Chicken
    chicken cytoskeletal proteins

    Aplicación

    Immunoblotting (1:5000-1:20000)

    Immunofluorescence (1:10000, see application references)



    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

    Advertencia

    Toxicity: Multiple Toxicity Values, refer to MSDS (O)

    Forma física

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    Reconstitución

    Following initial thaw, aliquot ascites and freeze (-20°C).

    Nota de análisis

    Positive Control
    All animal cell lines

    Otras notas

    Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

    Información legal

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Código de clase de almacenamiento

    11 - Combustible Solids

    Clase de riesgo para el agua (WGK)

    WGK 1

    Punto de inflamabilidad (°F)

    Not applicable

    Punto de inflamabilidad (°C)

    Not applicable


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