CC145

Rat Laminin

from rat bladder carcinoma, liquid, suitable for cell culture

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Propiedades

• Nombre del producto: Rat Laminin-5,
• origen biológico: rat
• Nivel de calidad: 100
• Ensayo: >95% (SDS-PAGE)
• Formulario: liquid
• fabricante / nombre comercial: Chemicon^®
• técnicas: cell culture | mammalian: suitable
• entrada: sample type neural stem cell(s); sample type induced pluripotent stem cell(s); sample type epithelial cells; sample type pancreatic stem cell(s); sample type: mouse embryonic stem cell(s); sample type mesenchymal stem cell(s); sample type hematopoietic stem cell(s)
• Nº de acceso UniProt: P70636
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: rat ... Lama5(140433)

Descripción

Descripción general

Laminin-5 is a basement membrane extracellular matrix macromolecule that provides an attachment substrate for both adhesion and migration in a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. It is a natural substrate for cells in contact with basement membranes, including epidermal and oral keratinocytes, gastro-intestinal tract, mammary and prostate epithelial cells. These cells, compared to fibronectin, collagen, or vitronectin, will adhere to Laminin-5 faster and spread to a larger extent. Laminin-5 can be used, in most applications, at coating concentrations in the 1 mg/ml or lower range, approximately 10-fold lower than most extracellular matrix macromolecules. Laminin-5 is attracting attention as a substrate that may stimulate carcinoma cell migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas. Receptors for Laminin-5 include integrins a3b1 and a6b4. The latter integrin is specialized in forming hemidesmosomes, which provide resistance to mechanical stress in many epithelia, including skin and gut. More recently, cell surface proteoglycans, such as syndecan-4, have been identified as receptors for Laminin-5. The structural and functional properties of Laminin-5 are well conserved across species. For instance, human cells adhere equally well to human and rat laminin-5. For most purposes, human and rat Laminin-5 are interchangeable, likely based on the fact that integrin receptor specificity is largely conserved across species.

Product Source: Rat bladder carcinoma cell line 804G {Koshikawa, N et al. (2000) J. Cell Biol. 148:615-624.

Aplicación

Recommended coating concentrations: 1 μg/mL for cell adhesion assays, 0.2 μg/mL for cell migration assays.

Optimal dilutions must be determined by end user

Forma física

Purified protein supplied in 230mM tris, pH 7.5, 0.007% Brij-35, 0.1% CHAPS containing no preservatives.

Almacenamiento y estabilidad

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Otras notas

Concentration: Variable

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable