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CC130

Sigma-Aldrich

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM®

Sinónimos:

S-protein, Serum-spreading factor, V75

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Nombre del producto

PluriSTEM-XF® Recombinant Vitronectin, Human pluripotent stem cell culture.

origen biológico

Escherichia coli
human

Nivel de calidad

100

Ensayo

≥92% (SDS-PAGE)

Formulario

liquid

fabricante / nombre comercial

PluriSTEM®

concentración

0.5 mg/mL

técnicas

cell attachment: suitable
cell culture | mammalian: suitable
cell culture | stem cell: suitable

entrada

sample type: human embryonic stem cell(s)

Nº de acceso UniProt

P04004

Condiciones de envío

dry ice

Descripción general

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Aplicación

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Calidad

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Forma física

Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.

Almacenamiento y estabilidad

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.

Información legal

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

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Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolos

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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