CBL171
Anti-Actin Antibody, smooth muscle, clone ASM-1
clone ASM-1, Chemicon®, from mouse
Sinónimos:
Alpha-actin-2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
ASM-1, monoclonal
reactividad de especies
chicken, mouse, horse, rat, human, bovine
fabricante / nombre comercial
Chemicon®
técnicas
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotipo
IgG2a
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ACTA2(59)
Descripción general
ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
Especificidad
Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.
This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).
Inmunógeno
Epitope: N-terminus
Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
Aplicación
Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Calidad
Evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Descripción de destino
43 kDa
Ligadura / enlace
Replaces: 04-1040
Forma física
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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