AP501B

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed

Chemicon^®, from goat

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Propiedades

• origen biológico: goat
• Nivel de calidad: 100
• conjugado: biotin conjugate
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• reactividad de especies: mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; immunofluorescence: suitable
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Especificidad

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Inmunógeno

Epitope: Mouse IgM, IgG, IgA

Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

Aplicación

Detect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.

Immunofluorescence: ≤1 μg/10E6 cells
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Forma física

Affinity Chromatography

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.

Almacenamiento y estabilidad

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable