AP180B

Donkey Anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed

1 mg/mL, Chemicon^®

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Propiedades

• origen biológico: donkey
• Nivel de calidad: 100
• conjugado: biotin conjugate
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• reactividad de especies: goat
• fabricante / nombre comercial: Chemicon^®
• concentración: 1 mg/mL
• técnicas: ELISA: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Especificidad

Specific for Goat IgG.

Aplicación

Donkey anti-Goat IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Goat IgG & has been published & validated for use in ELISA, FC, IF, IH & WB.

EIA: 1:20,000-1:400,000
Western blots using enzyme-conjugated streptavidin: 1:20,000-1:400,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000
Optimal working dilutions must be determined by end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Forma física

Immunoaffinity Purified and Immunodepletion

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.

Almacenamiento y estabilidad

Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Frases de peligro: H412
• Consejos de prudencia: P273 - P501
• Clasificaciones de peligro: Aquatic Chronic 3
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3