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Sigma-Aldrich

Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate

Chemicon®, from goat

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

alkaline phosphatase conjugate

forma del anticuerpo

F(ab′)2 fragment of affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

reactividad de especies

human

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
western blot: suitable

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG (H+L)

Especificidad

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.

Aplicación

Detect Human IgG using this Goat anti-Human IgG Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.
EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Ligadura / enlace

Replaces: MABN1055

Forma física

Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Serum

Almacenamiento y estabilidad

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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International journal of biochemistry and molecular biology, 11(1), 1-10 (2020-03-27)
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