ABT11

Anti-Fibroblast activation protein alpha (FAP) Antibody

from rabbit

• Sinónimos: fibroblast activation protein, alpha, fibroblast activation protein, alpha subunit, integral membrane serine protease, seprase, 170 kDa melanoma membrane-bound gelatinase, FAP
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: saturated ammonium sulfate (SAS) precipitated
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_004451
• Nº de acceso UniProt: Q12884
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FAP(2191)

Descripción

Descripción general

Fibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.

Especificidad

The antibody recognizes the extracellular domain of Fibroblast activation protein alpha

Inmunógeno

Epitope: Extracellular domain

Histidine-tagged recombinant protein corresponding to human FAP.

Aplicación

Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.

Immunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002).

Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).

Research Category
Cell Structure

Apoptosis & Cancer

Research Sub Category
ECM Proteins

Tumor Markers

Calidad

Evaluated by Western Blot in HEK-FAP xenograft tumor lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.

Descripción de destino

~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa It is possible that both bands may be obserbved in certain cell lines.

Forma física

Ammonium Sulfate

Format: Purified

Purified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HEK-FAP xenograft tumor lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1