ABS994

Anti-phospho-Glucocorticoid receptor Antibody, Ser226

from rabbit, purified by affinity chromatography

• Sinónimos: Nuclear receptor subfamily 3 group C member 1, glucocorticoid receptor, GR
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_000167
• Nº de acceso UniProt: P04150
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer226)
• Información sobre el gen: human ... NR3C1(2908)

Descripción

Descripción general

Glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1, and encoded by the gene name NR3C1/ GRL, is receptor for glucocorticoids (GC). GR has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. GR affects inflammatory responses, cellular proliferation and differentiation in target tissues. Recent studies have shown that GR may act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. In addition, GR is involved in chromatin remodeling, and may play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. Mutation in GR has been associated with Glucocorticoid resistance (GCRES): a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. GR is widely expressed. In the heart, GR is detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart.

Inmunógeno

Epitope: A region within the modulating domain

KLH-conjugated linear peptide corresponding to the domain of: Modulating region of Human Glucocorticoid receptor.

Aplicación

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

This Anti-phospho-Glucocorticoid receptor antibody is validated for use in Western Blotting for the detection of phospho-Glucocorticoid receptor.

Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in transfected U2OS cell lysate (Chen, W., et al. Mol Endocrinol, August 2008, 22(8):1754–1766)
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in rat hepatoma cell lysate (Blind,R., et al. Journal of Steroid Biochemistry & Molecular Biology 109 (2008) 150–157)

Calidad

Evaluated by Western Blotting on transfected HEK293 cell lysate

Western Blotting Analysis: 1 µg/mL dilution of this antibody detected phosphorylated glucocorticoid receptor in 6 µg of transfected HEK293 cell lysate

Descripción de destino

~85 kDa observed

Forma física

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable