ABS65

Anti-CPT1A Antibody

serum, from rabbit

• Sinónimos: Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rat, mouse
• técnicas: western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_001867
• Nº de acceso UniProt: P50416
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Cpt1A(12894)

Descripción

Descripción general

Carnitine palmitoyltransferase 1A (CPT1A, CPT1-L, or L-CPT1) is an hepatic enzyme that, together with CPT2 and carnitine-acylcarnitine translocase, mediates the oxidation of long chain fatty acids to produce energy, in periods of fasting. CPT1A is localized in the outer mitochondrial membrane and catalyzes the addition of carnitine to long-chain fatty acids thereby enabling effective transport of these molecules to the inner mitochondrial membrane where they are metabolized. CPT1A may function as part of a complex consisting of the acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC) and is regulated by malonyl-CoA. Deficiencies in CPT1A result in hypoketotic hypoglycemia.

Inmunógeno

GST-tagged recombinant protein corresponding to rat CPT1A.

Aplicación

Detect CPT1A using this Anti-CPT1A Antibody validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Calidad

Evaluated by Western Blot in mouse liver tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected CPT1A on 10 µg of mouse liver tissue lysate.

Descripción de destino

~78 kDa observed. An uncharacterized band appears at ~32 kDa in some lysates. The calculated molecular weight is 88 kDa, however CPT1A has been shown as a ~78 kDa band in western blots (Tan, L., et al (2011). J Inherit Metab Dis. 34:443–447.)

Forma física

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Mouse liver tissue lysate

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1