ABS222

Anti-DEPTOR Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_073620
• Nº de acceso UniProt: Q8TB45
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... DEPTOR(64798)

Descripción

Descripción general

DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).

Especificidad

Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.

This antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR.

Inmunógeno

Epitope: DEP domain-containing mTOR-interacting protein DEPTOR.

KLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR.

Aplicación

Anti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB.

Research Category
Metabolism

Research Sub Category
PI3K, Akt, & mTOR Signaling

Calidad

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.

Descripción de destino

~46 kDa observed.

Ligadura / enlace

Replaces: 09-463

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HEK293 cell lyaste

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable