ABS2203

Anti-UBE2A

from rabbit, purified by affinity chromatography

• Sinónimos: Ubiquitin-conjugating enzyme E2A, EC 2.3.2.23, E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: polyclonal
• application: IHC; WB
• reactividad de especies: mouse, human
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• citations: 1

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology)
• envase: antibody small pack of 25 μg
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_003327
• Nº de acceso UniProt: P49459
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... UBE2A(7319)

Descripción

Descripción general

Ubiquitin-conjugating enzyme E2 A (UniProt: P49459; also known as EC 2.3.2.23; E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A) is encoded by the UBE2A (also known as RAD6A) gene (GeneID: 7319) in human. UBE2A belongs to the ubiquitin-conjugating enzyme family and accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1, it plays a role in transcription regulation by catalyzing the mono-ubiquitination of histone H2B at Lys-120 that provides a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, and telomeric silencing. It is also a prerequisite for H3K4me and H3K79me formation. Defects in UBE2A gene have been linked to mental retardation, X-linked, syndromic, Nascimento-type mental retardation that is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior.

Especificidad

This rabbit polyclonal antibody detects Ubiquitin-conjugating enzyme E2 A (UBE2A) in HeLa cells and Mouse Kidney.

Inmunógeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Ubiquitin-conjugating enzyme E2 A (UBE2A).

Aplicación

Anti-UBE2A Antibody, Cat. No. ABS2203, is a rabbit polyclonal antibody that detects UBE2A and has been tested for use Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A 1:500 dilution from a representative lot detected UBE2A in mouse kidney tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE2A in human pancreas, human cervical cancer, and human uterus tissues.

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected UBE2A in HeLa cell lysate.

Descripción de destino

~17 kDa observed; 17.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable