ABS194

Anti-phospho PDHE1-A type I (Ser300) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_000275
• Nº de acceso UniProt: P08559
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer300)
• Información sobre el gen: human ... PDHA1(5160)

Descripción

Descripción general

In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.

Especificidad

This antibody recognizes PDHE1-A type I phosphorylated at Ser300.

Inmunógeno

Epitope: Phosphorylated Ser300

KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.

Aplicación

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.

This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).

Calidad

Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.

Descripción de destino

~43 kDa observed

Nota de análisis

Control
Dichloroacetate untreated and treated HEK 293 cell lysates

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable