ABS1653

Anti-GSTT1 Antibody

serum, from rabbit

• Sinónimos: Glutathione S-transferase theta-1, Glutathione S-transferase T1-1, GST class-theta-1, GST T1-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rat, human, mouse
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_000844
• Nº de acceso UniProt: P30711
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GSTT1(2952)

Descripción

Descripción general

Glutathione S-transferase theta-1 (EC 2.5.1.18; UniProt P30711; also known as Glutathione S-transferase T1-1, GST class-theta-1, GST T1-1) is encoded by the GSTT1 gene (Gene ID 2952) in human. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTT1 exists as a homodimer, the subunit is referred to as subunit 5 in rat species, and T1-1 theta subunit in human.

Especificidad

Expected to react with both spliced isoforms reported by UniProt (P30711). Note that not all human samples are positive for GSTT1 expression. It has been estimated that approx. 18% of the Caucasian, and as much as 60% of the Chinese and Korean populations are GSTT1-null (Katoh, T., et al. (1996) Carcinogenesis. 17(9):1855-1859; Lear, J.T., et al. (1996) Carcinogenesis. 17(9):1891-1896;).

Inmunógeno

His-tagged recombinant full-length human GSTT1.

Aplicación

Anti-GSTT1 Antibody is an antibody against GSTT1 for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Signaling

Research Sub Category
Oxidative Stress

Western Blotting Analysis: A representative lot detected recombinant human and murine GSTT1, as well as endogenous GSTT1 in human and mouse liver samples (Sherratt, P.J., et al. (2002). Toxicol. Appl. Pharmacol. 179(2):89-97),
Western Blotting Analysis: A representative lot detected significantly upregulated GSTT1 levels in liver cytosolic preparations from rats on a selenium-deficient diet (McLeod, R., et al. (1997). Cancer Res. 57(19):4257-4266).
Western Blotting Analysis: A representative lot detected recombinant human GSTT1, as well as endogenous human GSTT1 in multiple tissue samples from four deseased individuals (Sherratt, P.J., et al. (1997). Biochem. J. 326 (Pt 3):837-846).
Immunohistochemistry Analysis: A representative lot detected differential GSTT1 immunoreactivity among liver cells in formalin-fixed, paraffin-embedded human and mouse liver tissue sections (Sherratt, P.J., et al. (2002). Toxicol. Appl. Pharmacol. 179(2):89-97).

Calidad

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected glutathione S-transferase theta-1 (GSTT1) in 10 µg of human liver tissue lysate.

Descripción de destino

~27 kDa observed (monomer). 27.3 kDa (isoform 1) and 13.5 kDa (isoform 2) calculated.

Forma física

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable