ABS1644

Anti-DsbA-L

from rabbit

• Sinónimos: Glutathione S-transferase kappa 1, EC:2.5.1.18, GST 13-13, GST class-kappa, GSTK1-1, mGSTK1, Glutathione S-transferase subunit 13
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• forma del anticuerpo: unpurified
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, human
• envase: antibody small pack of 25 μL
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_083831
• Nº de acceso UniProt: Q9DCM2
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Gstk1(76263)

Descripción

Descripción general

Glutathione S-transferase kappa 1 (UniProt: Q9DCM2; also known as EC:2.5.1.18, GST 13-13, GST class-kappa, GSTK1-1, mGSTK1, Glutathione S-transferase subunit 13, DsbA-L) is encoded by the Gstk1 gene (Gene ID: 76263) in murine species. DsbA-L is a disulfide bond A oxidoreductase like homodimeric mitochondrial protein that is predominantly expressed in heart, kidney, liver and skeletal muscle. It is also highly expressed in adipose tissue and its expression level is negatively correlated with obesity in mice and humans. Overexpression of DsbA-L is shown to promote adiponectin multimerization. Suppression of DsbA-L expression can selectively reduce adiponectin levels and secretion in 3T3-L1 adipocytes. Expression of DsbA-L in 3T3-L1 adipocytes is shown to be stimulated by the insulin sensitizer rosiglitazone and inhibited by TNFalpha. Adiponectin expression is reduced in both human obesity and mouse models of genetic obesity. DsbA-L is also shown to co-localize with the endoplasmic reticulum (ER) marker protein disulfide isomerase and it interacts with the ER chaperone protein Ero1-L in 3T3-L1 adipocytes. Deletion of the first 6 amino acid residues at the N terminus greatly impaired DsbA-L localization in the ER. (Ref.: Liu, M et al. (2015). J Biol Chem. 290(16):10143-8).

Especificidad

This rabbit polyclonal antibody detects DsbA-L (Glutathione S-transferase kappa 1) in human and mouse cells.

Inmunógeno

Epitope: unknown

Myc-tagged full length recombinant protein from mouse.

Aplicación

Anti-DsbA-L, Cat. No. ABS1644, is a rabbit polyclonal antibody that detects Glutathione S-transferase kappa 1 (DsbA-L) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected DsbA-L in A431, HUVEC, NIH/3T3 cells.

Western Blotting Analysis: A representative lot detected DsbA-L in mouse brain, mouse kidney, and mouse liver (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).

Immunoprecipitation Analysis: A representative lot detected DsbA-L in 3T3-L1 adipocytes (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).

Immunocytochemistry Analysis: A representative lot detected DsbA-L in adipocytes on differentiation day 7 were used for the following studies. confocal immunofluorescence study of DsbA-L co-localization with the mitochondrial marker (MitoTracker) and the ER marker (PDI) in 3T3-L1 adipocytes (Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).

Research Category
Signaling

Calidad

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DsbA-L in 10 µg of mouse kidney tissue lysate.

Descripción de destino

~28 kDa observed; 25.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.

Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de riesgo para el agua (WGK): WGK 1