ABN427

Anti-RalDH3 (ALDH1A3)

from rabbit, purified by affinity chromatography

• Sinónimos: Aldehyde dehydrogenase family 1 member A3, Aldehyde dehydrogenase 6, RALDH-3, RalDH3, Retinaldehyde dehydrogenase 3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, mouse, human
• técnicas: immunofluorescence: suitable; western blot: suitable
• Nº de acceso NCBI: NP_444310
• Nº de acceso UniProt: Q9JHW9
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ALDH1A3(220)

Descripción

Descripción general

Aldehyde dehydrogenase family 1 member A3 (EC 1.2.1.5; UniProt Q9JHW9; also known as Aldehyde dehydrogenase 6, RALDH-3, RalDH3, Retinaldehyde dehydrogenase 3) is encoded by the Aldh1a3 (also known as Aldh6, Raldh3) gene (Gene ID 56847) in murine species. All-trans-retinoic acid (atRA) is a small molecule metabolite of retinol, also known as vitamine A, that plays an important role in the growth and development of many major organ systems, including the postnatal eye growth. The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA. While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs). The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns. In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina. RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera. RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE. RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.

Inmunógeno

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of mouse RalDH3.

Aplicación

Anti-RalDH3 (ALDH1A3), Cat. No. ABN427, is a highly specific rabbit polyclonal antibody that targets RalDH3/ALDH1A3 and has been tested in Immunofluorescence and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilutioin from a representative lot detected RalDH3 in 10 µg of human retina tissue lysate.

Immunofluorescence Analysis: A 1:150 dilutioin from a representative lot immunostained trabecula analge of the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistry (Courtesy of Anna Ashton, University of Aberdeen).

Western Blotting Analysis: A 1:1,500 dilutioin from a representative lot detected RalDH3 in 50 µg of new born (postnatal P0) Sprague Dawley (SD) rat whole eye tissue lysate, but not in adult SD rat brain lateral cortex and pons tissue lysates (Courtesy of Anna Ashton, University of Aberdeen).

Calidad

Evaluated by Western Blotting in mouse retina tissue lysate.

Western Blotting Analysis: A 1:500 dilutioin of this antibody detected RalDH3 in 10 µg of mouse retina tissue lysate.

Descripción de destino

~53 kDa observed. 55.98/56.03/56.04 kDa (human/mouse/rat) calculated (Met1 removed).

Forma física

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable