ABN2290

Anti-LHFPL5

• Sinónimos: LHFPL tetraspan subfamily member 5 protein, Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: N/A, polyclonal
• mol peso: calculated mol wt 24.2 kDa
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey
• envase: antibody small pack of 100 μL
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG
• secuencia del epítopo: C-terminal
• Nº de acceso Protein ID: NP_872354
• Nº de acceso UniProt: Q8TAF8
• temp. de almacenamiento: -10 to -25°C
• Información sobre el gen: human ... LHFPL5(222662)

Descripción

Descripción general

LHFPL tetraspan subfamily member 5 protein (UniProt: Q8TAF8; also known as Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia) is encoded by the LHFPL5 (also known as TMHS) gene (Gene ID: 222662) in human. LHFPL5 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. Its localization to the plasma membrane requires the presence of protocadherin related 15 (PCDH15). LHFPL5 is recognized as a critical component of the mechano-transduction complex in inner-ear hair cells. It is shown to physically interact with and stabilize transmembrane channel-like protein 1 (TMC1). The C-terminal half of LHFPL5 is involved in this interaction. The N-terminal half is involved in its dimerization and interaction with PCDH15. Mutations in LHFPL5 gene are known to cause autosomal recessive deafness that is characterized by non-syndromic sensorineural hearing loss from damage to the neural receptors of the inner ear. Knockout of LHFPL5 in murine models is shown to result in TMC1 reduction in the hair bundles of auditory cells. (Ref.: Yu, X., et al. (2020). Proc, Natl. Acad. Sci. USA. 117(47); 29894-29903).

Especificidad

This rabbit polyclonal antibody detects LHFPL tetraspan subfamily member 5 protein (LHFPL5). It targets an epitope within 20 amino acids from the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human LHFPL tetraspan subfamily member 5 protein (LHFPL5).

Aplicación

Quality Control Applications

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LHFPL5 in HeLa cells.

Tested applications

Western Blotting Analysis: A representative lot detected LHFPL5 in Western Blotting applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LHFPL5 in HEK293 cells transfected with LHFPL5 (TMHS) protein.

Immunocytochemistry Analysis: A representative lot detected LHFPL5 in Immunocytochemistry applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Forma física

Rabbit polyclonal antibody in 0.02 mg/mL sodium azide.

Almacenamiento y estabilidad

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1