ABN2242

Anti-Vangl2

from rabbit, purified by affinity chromatography

• Sinónimos: Vang-like protein 2 (UniProt: Q9ULK5, also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, mouse
• reactividad de especies (predicha por homología): bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• envase: antibody small pack of 25 μg
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_065068
• Nº de acceso UniProt: Q9ULK5
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... VANGL2(57216)

Descripción

Descripción general

Vang-like protein 2 (UniProt: Q9ULK5; also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2) is encoded by the VANGL2 (also known as KIAA1215, STB1) gene (Gene ID: 57216) in human. Vangl2 is a homodimeric multi-pass membrane protein that plays a role in the control of early morphogenesis, patterning of both axial midline structures and the development of neural plate. It can also form heterodimer with VANGL1. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Vang-like protein 2 is reported to bind to Dvl to cell-autonomously promote efficient Dvl plasma membrane recruitment, a pre-requisite for planar cell polarity activation. At the same time, it can inhibit Dvl from interacting with its downstream effector (Dishevelled associated activator of morphogenesis 1 (Daam1), and functionally suppresses Dvl - Daam1 cascade. Mutations in VANGL2 gene cause neural tube defects that results in defective neural tube closure during the first trimester of pregnancy. (Ref.: Seo, HS et al. (2017). Hum. Mol. Genet. 26(11); 2053-2061).

Especificidad

This rabbit polyclonal antibody detects Vang-like protein 2 in human and murine cells. It targets an epitope with in 15 amino acids from the N-terminal region.

Inmunógeno

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Vang-like Protein 2.

Aplicación

Anti-Vangl2, Cat. No. ABN2242, is a highly specific rabbit polyclonal antibody that targets Vang-like protein 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vangl2 in HT1080 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Vangl2 in human tonsil and mouse embryonic spinal cord tissue sections.

Research Category
Neuroscience

Calidad

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Vangl2 in HepG2 cell lysate.

Descripción de destino

~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1