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ABE572

Sigma-Aldrich

Anti-N6-methyladenosine (m6A) Antibody

from rabbit

Sinónimos:

m6A

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies (predicha por homología)

all

técnicas

ChIP: suitable
RIP: suitable
dot blot: suitable

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse.

Inmunógeno

BSA-conjugated N6-methyladenosine.

Aplicación

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in RNA containing N6-methyladenosine (Dominissini, D., et al. (2012). Nature. 485(7397):201-206.; Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).

(RNA) Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in methylated RNA containing N6-methyladenosine (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).

Dot Blot Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in a panel of modified oligonucleotides (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.; Jia, G., et al. (2011). Nat Chem Biol. 7(12):885-887.).
Detect N6-methyladenosine (m6A) using Anti-N6-methyladenosine (m6A) Antibody validated for use in Dot Blot, Chromatin IP (ChIP) & RNA Binding Protein IP (RIP).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Calidad

Evaluated by Dot Blot in oligomers containing N6-methyladenosine.

Dot Blot Analysis: 1 µg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.

Forma física

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Karolos Douvlataniotis et al.
Science advances, 6(12), eaay3335-eaay3335 (2020-03-25)
N6-methyladenine (6mdA) is a widespread DNA modification in bacteria. More recently, 6mdA has also been characterized in mammalian DNA. However, measurements of 6mdA abundance and profiles are often very dissimilar between studies, even when performed on DNA from identical mammalian
Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons.
Meyer, Kate D, et al.
Cell, 149, 1635-1646 (2012)
Xiaoling Liu et al.
Analytical chemistry, 90(9), 5546-5551 (2018-04-14)
N6-methyladenine (6mA) is a rediscovered DNA modification in eukaryotic genomes. To explore the distribution and functions of 6mA, it is of paramount option to use immunoprecipitation to select 6mA-containing DNA fragments for genome-wide sequencing. Presumably, most of the 6mA-free fragments
Jiyu Tong et al.
Science advances, 7(18) (2021-04-30)
m6A RNA modification is implicated in multiple cellular responses. However, its function in the innate immune cells is poorly understood. Here, we identified major m6A "writers" as the top candidate genes regulating macrophage activation by LPS in an RNA binding
Guifang Jia et al.
Nature chemical biology, 7(12), 885-887 (2011-10-18)
We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of
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