ABE558

Anti-Ku70 Protein Antibody

from rabbit

• Sinónimos: X-ray repair cross-complementing protein 6, 5′-deoxyribose-5-phosphate lyase Ku70, 5′-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subuni
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_001460
• Nº de acceso UniProt: P12956
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... XRCC6(2547)

Descripción

Descripción general

Lupus Ku autoantigen protein p70 (Ku70) is also known as X-ray repair cross-complementing protein 6 (XRCC6), 5′-dRP lyase Ku70, CTC box-binding factor 75 kDa subunit (CTC75, CTCBF) or Thyroid-lupus autoantigen (TLAA). Ku70 is a single stranded DNA-dependent ATP-dependent helicase that plays a role in chromosome translocation and DNA non-homologous end joining (NHEJ) required for double-strand break repair and recombination. The XRCC5/6 (Ku80/Ku70) dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK, and that dimer, together with APEX1, acts as a negative regulator of transcription. Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to XRCC5 (Ku80) and XRCC6 (Ku70).

Especificidad

This antibody recognizes Ku70 Protein near N-Terminus.

Inmunógeno

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to human Ku70 Protein near N-Terminus.

Aplicación

Detect Ku70 Protein using this rabbit polyclonal antibody, Anti-Ku70 Protein Antibody validated for use in western blotting, IP & IHC (Paraffin).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ku70 Protein in 0.5 mg of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ku70 Protein in human prostate carcinoma cells and human testis tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ku70 Protein in 10 µg of HeLa cell lysate.

Descripción de destino

~70 kDa observed

Ligadura / enlace

Replaces: AB1358

Forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable