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Merck
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Key Documents

ABE547

Sigma-Aldrich

Anti-ALKBH5 Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

RNA demethylase ALKBH5, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human

reactividad de especies (predicha por homología)

bovine (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology)

técnicas

immunoprecipitation (IP): suitable
western blot: suitable

Nº de acceso NCBI

NP_060228.3

Nº de acceso UniProt

Q6P6C2

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ALKBH5(54890)

Descripción general

RNA demethylase ALKBH5 is also known as Alkylated DNA repair protein alkB homolog 5 and Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5. ALKBH5 is a dioxygenase that demethylates RNA, specifically N(6)-methyladenosine (m6A) RNA, which affects mRNA processing and export and is needed for spermatogenesis. ALKBH5 has the highest expression in lung, testis, pancreas, spleen and ovary, but is widely expressed across other tissues.

Inmunógeno

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ALKBH5.

Aplicación

Detect ALKBH5 using this rabbit polyclonal antibody, Anti-ALKBH5 Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot detected ALKBH5 from 500 µg of MCF-7 cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Calidad

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ALKBH5 in 10 µg of MCF-7 cell lysate.

Descripción de destino

~52 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forma física

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ye Yuan et al.
Cell death & disease, 12(1), 60-60 (2021-01-13)
ALKBH5 is the main enzyme for m6A-based demethylation of RNAs and it has been implicated in many biological and pathophysiological processes. Here, we aimed to explore the potential involvement of ALKBH5 in osteosarcoma and decipher the underlying cellular/molecular mechanisms. We
Zhaofeng Li et al.
Cell death & disease, 12(6), 578-578 (2021-06-06)
N6-methyladenosine (m6A) modification is widespread in messenger RNAs and increasing evidence suggests the crucial roles of m6A in cell differentiation and tissue development. However, whether m6A modulates the osteogenic differentiation of mesenchymal stem cells (MSCs) has not been fully elucidated.
Wei Song et al.
Cell death discovery, 8(1), 25-25 (2022-01-16)
Long noncoding RNAs (lncRNAs) have crucial functions in the tumorigenesis and metastasis of cancers. N6-methyladenosine (m6A) modification of RNA is an important epigenetic regulatory mechanism in various malignancies. Nevertheless, the mechanism of m6A-modified lncRNA in diffuse large B cell lymphoma
Sicong Zhang et al.
Cancer cell, 31(4), 591-606 (2017-03-28)
The dynamic and reversible N6-methyladenosine (m6A) RNA modification installed and erased by N6-methyltransferases and demethylases regulates gene expression and cell fate. We show that the m6A demethylase ALKBH5 is highly expressed in glioblastoma stem-like cells (GSCs). Silencing ALKBH5 suppresses the

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