ABE2873

Anti-NUDT21

from rabbit

• Sinónimos: Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor 25 kDa subunit, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_008937
• Nº de acceso UniProt: O43809
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... NUDT21(11051)

Descripción

Descripción general

Cleavage and polyadenylation specificity factor subunit 5 (UniProt: O43809; also known as Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit) is encoded by the NUDT21 (also known as CFIM25, CPSF25, CPSF5) gene (Gene ID: 11051) in human. CFlm25 is a homodimeric protein that is a component of the cleavage factor Im (CFIm) complex, which plays a key role in pre-mRNA 3′-processing. It is involved in association with CPSF6 or CPSF7 in pre-MRNA 3′-end poly(A) site cleavage and poly(A) addition. The interaction of CFlm25 with the RNA is one of the earliest steps in the assembly of the 3′ end processing complex and facilitates the recruitment of other processing factors. It contains one Nudix hydrolase domain (aa 76-201). The region containing aa 2-147 is considered to be essential for RNA-binding. It can be acetylated by p300/CBP and acetylation results in a reduction in its interaction with Poly(A) polymerase alpha (PAPAO).

Especificidad

This rabbit polyclonal antibody detects Cleavage and polyadenylation specificity factor subunit 5 (CFIm25) in human cells.

Inmunógeno

Epitope: unknown

His-tagged full length recombinant human Cleavage and polyadenylation specificity factor subunit 5 (CFIm25).

Aplicación

Anti-NUDT21, Cat. No. ABE2873, is a highly specific rabbit polyclonal antibody that targets Cleavage and polyadenylation specificity factor subunit 5 and has been tested in Immunoprecipitation and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected NUDT21 in RNase A-treated HeLa whole-cell extract after IP (Katahira, J., et. al. (2013). Nucleic Acid Res. .41(14):7060-72).

Immunoprecipitation Analysis: A representative lot detected NUDT21 in Co-immunoprecipitation with FLAG-tagged CF I cell lysates (Shimazu, T., et. al. (2007). J Biol Chem. 282(7):4470-8).

Calidad

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NUDT21 in 10 µg of Caco-2 cell lysate.

Descripción de destino

~25 kDa observed; 26.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable