ABE2621

Anti-CEP164

from rabbit

• Sinónimos: Centrosomal protein of 164 kDa
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_055771
• Nº de acceso UniProt: Q9UPV0
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CEP164(22897)

Descripción

Descripción general

Centrosomal protein of 164 kDa (UniProt: Q9UPV0; also known as Cep164) is encoded by the CEP164 (also known as KIAA1052, NPHP15) gene (Gene ID: 22897) in human. Cep164 is expressed in several cell lines and it plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Cep164 localizes specifically to very distally located appendage structures on the mature centriole from which PC formation originates and it persists at centrioles throughout mitosis. Hence, it can serve as a marker for distal appendages on mature centrioles or basal bodies. Cep164 also plays a vital role in G2/M checkpoint and nuclear divisions. It is a key participant in the DNA damage-activated ATR/ATM signaling cascade and is required for the proper phosphorylation of H2AX, replication protein A, and check point kinases 1 and 2. Its phosphorylation at Ser186 is induced upon DNA-damage caused by treatment with IR irradiation, UV irradiation, hydroxyurea or amphidicolin. Mutations in CEP164 gene are known to cause nephronophthisis, a disease characterized by blindness in childhood, facial dysmorphism, bronchieclasis, liver failure and progression to end-stage renal failure.

Especificidad

This polyclonal antibody detects Centrosomal protein of 164 kDa in human. It targets an epitope within the first 298 amino acids from the N-terminal region.

Inmunógeno

Epitope: N-terminus

His-taggged recombinant fragment of the first 298 amino acids from human Centrosomal protein of 164 kDa (CEP164).

Aplicación

Anti-CEP164, Cat. No. ABE2621, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 164 kDa and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected CEP164 in U2OS cells and hTERT-RPE1 cells that were serum starved for 48 hours (Graser, S., et. al. (2007). J Cell Biol. 179(2):321-30; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).

Western Blotting Analysis: A representative lot detected CEP164 in total lysates of HeLa S3, U2OS, and 293T cells as well as 293T cells overexpressing myc-Cep164 (Graser, S., et. al. (2007). J Cell Biol. 179(2):321-30).

Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CEP164 in 10 µg of U2OS cell lysate.

Descripción de destino

~200 kDa observed; 164.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1