ABE1800

Anti-La-Related Protein 1

from rabbit, purified by affinity chromatography

• Sinónimos: La ribonucleoprotein domain family member 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• envase: antibody small pack of 25 μg
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_056130
• Nº de acceso UniProt: Q6PKG0
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... LARP1(23367)

Descripción

Descripción general

La-related protein 1 (UniProt: Q6PKG0; also known as La ribonucleoprotein domain family member 1) is encoded by the LARP1 (also known as KIAA0731, LARP) gene (Gene ID: 23367) in human. La-related protein 1 is a cytoplasmic RNA-binding protein that promotes translation of specific classes of mRNAs downstream of the mTORC1 complex. It contains 1 HTH La-type RNA-binding domain and it associates with the mRNA 5′cap in an mTOR-dependent manner and associates with mRNAs containing a 5′ terminal oligopyrimidine (5′TOP) motif, which is present in mRNAs encoding for ribosomal proteins and several components of the translation machinery. La-related protein 1 is also shown to associates with actively translating ribosomes via interaction with polyadenylate-binding protein 1 (PABPC1/PABP) and stimulates translation of mRNAs containing a 5′TOP, thereby regulating cell growth and proliferation. It is shown to be up-regulated in a number of hepatocellular carcinoma cell lines and liver cancer lesions, as well as in patients with hepatocellular carcinoma with a lower survival rate. (Ref.: Fonseca, BD et al. (2015). J. Biol. Chem. 290(26):15996-6020).

Inmunógeno

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human La-related protein 1.

Aplicación

Anti-La-Related Protein 1 Antibody, Cat. No. ABE1800, is a highly specific rabbit polyclonal antibody that targets La-related protein 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected La-Related Protein 1 in HEK293 cells.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected La-Related Protein 1 in human cerebellum, human tonsil, and human pancreas tissues.

Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected La-Related Protein 1 in 10 µg of HEK293 cell lysate.

Descripción de destino

~123 kDa observed; 123.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1