ABE1359

Anti-PHD finger protein 14 (PHF14)

from rabbit, purified by affinity chromatography

• Sinónimos: PHF14
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• envase: antibody small pack of 25 μL
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_055475.2
• Nº de acceso UniProt: O94880
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PHF14(9678)

Descripción

Descripción general

PHD finger protein 14 (UniProt: O94880) is encoded by the PHF14 (also known as KIAA0783) gene (Gene ID: 9678) in human. PHF14 is a member of the PHD finger protein family that serves as a chromatin-binding protein. It contains four putative PHD fingers, two coiled-coil regions, and four zinc fingers. PHF14 is highly conserved from C. elegans to humans and is considered as a transcriptional regulator of target gene expression. PHF14 has been identified as a regulator of mesenchyme growth in embryonic tissues. Two isoforms of PHF14 have been described that are produced by alternative splicing. PHF14 interacts with histones via its PHD1 and PHD3 domains indicating its role in epigenetic regulation. It is involved in chromatin-mediated transcriptional regulation and chromatin dynamics. During cell division, PHF14 is shown to be directly bound to and co-localized with KIF4A to form a functional complex. PHF14 is shown to be overexpressed in lung cancer and this overexpression is associated with poor prognosis of non-small cell lung carcinoma. (Ref.: Zhang, L., et al. (2017). Oncotarget. 8(12); 19684-19698; Yang, B., et al. (2017). Sci Rep. 7; 39888).

Especificidad

This rabbit polyclonal antibody detects PHD Finger Protein 14 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human PHD finger protein 14.

Aplicación

Anti-PHD finger protein 14 (PHF14) Antibody, Cat. No. ABE1359, is a highly specific rabbit polyclonal antibody that detects PHD finger protein 14 and has been tested for use in Immunocytochemistry and in Western Blotting.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PHD finger protein 14 (PHF14) in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PHD finger protein 14 (PHF14) in HEK293 cell lysate.

Descripción de destino

100.0 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable