ABD71

Anti-FOXC1 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3, FREAC-3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001444
• Nº de acceso UniProt: Q12948
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FOXC1(2296)

Descripción

Descripción general

Forkhead box protein C1 (FOXC1) is a member of the family of forkhead transcription factors. These proteins are characterized by the forkhead domain--a 110 amino acid domain that binds to DNA molecules. FOXC1 has been implicated in the development of the eye; disruption of the FOXC1 gene has been linked to a number of glaucoma-related diseases such as Axenfeld-Rieger syndrome.

Especificidad

Other homologies: Rat (71% sequence homology).

Inmunógeno

KLH-conjugated linear peptide corresponding to human FOXC1.

Aplicación

Anti-FOXC1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXC1 also known as Forkhead box protein C1, Forkhead-related protein FKHL7, FREAC-3 & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot Analysis: A representative lot of this antibody detected FOXC1 in HeLa, HEK293, THP1, and MDA-MB-468 cell lysates, and in human bone marrow tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FOXC1 in HeLa cells. This antibody positively stains the nucleus, with some cytoplasm staining. This staining pattern has also been observed by an independent laboratory (Berry, F. B., et al. (2002). J Biol Chem. 277(12):10292-10297.).

Calidad

Evaluated by Western Blot in THP1 cell lysate.

Western Blot Analysis: 0.025 µg/mL of this antibody detected FOXC1 in 10 µg of THP1 cell lysate. No Tween^® reagent was used in the wash buffers and in the primary and secondary antibody dilution buffers.

Descripción de destino

~75 kDa observed. Uniprot describes a molecular weight at ~57 kDa This protein may be observed at ~65 kDa in some cell lysates. (Tamimi, Y., et al. (2006). Hum Mol Genet. 15(21): 3229-3240.).

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
THP cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

TWEEN is a registered trademark of Croda International PLC

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable