ABC924

Anti-CtBP-2 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: C-terminal-binding protein 2, CtBP-2, CtBP2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), horse (based on 100% sequence homology), sheep (based on 100% sequence homology), mouse (based on 100% sequence homology)
• técnicas: immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001077383
• Nº de acceso UniProt: P56545
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CTBP2(1488)

Descripción

Descripción general

C-terminal-binding protein 2 (UniProt P56545; also known as CtBP2) is a nuclear protein encoded by the CTBP2 gene (Gene ID 1488) in human. CtBP1 and CtBP2 are closely related proteins that are encoded by the vertebrate genome and perform both unique and duplicative functions. Upon activation by nicotinamide adenine dinucleotide binding to their dehydrogenase domains, CtBP1 and CtBP2 exert transcriptional repression activity primarily via recruitment of a corepressor complex that consists of histone deacetylases (HDACs) and histone methyltransferases, although CtBPs can also repress transcription through HDAC-independent mechanisms. A critical function of CtBPs lies in the transcriptional repression of pro-apoptotic genes such as Bax, Puma, Bik, and Noxa. CtBP2 is essential for proper development of the nervous system. Acetylation by histone acetyl transferase p300 regulates CtBP2 activity and subcellular localization, while microenvironmental oxygen levels influences the dimerization, acetylation, and thereby the activity of CtBP2 in proliferating neural stem cells (NSCs).

Especificidad

Expected to react with spliced variant Ribeye (isoform 2).

Inmunógeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human CtBP-2.

Aplicación

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CtBP-2 in human breast cancer cells, human kidney, human ovarian cancer cells, and human prostate cancer cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-CtBP-2 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of CtBP-2 .

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected CtBP-2 in 10 µg of HeLa cell lysate.

Descripción de destino

~50 kDa observed. 48.9 kDa (Isoform 1; calculated); 109.2 kDa (Isoform 2/Ribeye; calculated)." ==> ~50 kDa observed. 48.9 kDa (Isoform 1; calculated); 109.2 kDa (Isoform 2/Ribeye; calculated).

Forma física

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable