AB9876
Anti-Stargazin Antibody
Chemicon®, from rabbit
Sinónimos:
Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
rat, mouse
fabricante / nombre comercial
Chemicon®
técnicas
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
mouse ... Cacng2(12300)
rat ... Cacng2(84347)
Especificidad
Stargazin [Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)]. By Western blot the antibody recognizes bands at ~35 kDa and ~55 kDa on Triton soluble fraction of rat brain. The antibody reacts dominantly with TARP gamma-8 and weakly with gamma-3 and gamma-4. Stargazin is expressed strongly in the hippocampus and cerebellum.
Inmunógeno
GST fusion protein from rat stargazin, cytoplasmic domain.
Aplicación
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Stargazin Antibody is validated for use in IP, WB, IC, IH for the detection of Stargazin.
Western blot: 0.1 μg/mL on Triton soluble fraction of rat brain. Suggested dilution buffer is TBS-T and blocking buffer is 3% skim milk. Suggested incubation time is 3 hours to overnight at room temperature.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.
Forma física
Format: Purified
ImmunoAffinity Purified
Liquid in 130 mM Tris-Cl, 87 mM Glycine, pH 7.4.
Almacenamiento y estabilidad
Maintain at -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze thaw cycles. The antibody may be kept at 2-8°C for 1-3 weeks after thawing.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk.
Ma, TM; Paul, BD; Fu, C; Hu, S; Zhu, H; Blackshaw, S; Wolosker, H; Snyder, SH
The Journal of Biological Chemistry null
Susumu Tomita et al.
The Journal of cell biology, 161(4), 805-816 (2003-05-29)
Functional expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in cerebellar granule cells requires stargazin, a member of a large family of four-pass transmembrane proteins. Here, we define a family of transmembrane AMPA receptor regulatory proteins (TARPs), which comprise stargazin, gamma-3, gamma-4
Susumu Tomita et al.
Science (New York, N.Y.), 303(5663), 1508-1511 (2004-03-06)
Activity-dependent plasticity in the brain arises in part from changes in the number of synaptic AMPA receptors. Synaptic trafficking of AMPA receptors is controlled by stargazin and homologous transmembrane AMPA receptor regulatory proteins (TARPs). We found that TARPs were stable
Susumu Tomita et al.
Neuron, 45(2), 269-277 (2005-01-25)
Synaptic plasticity involves protein phosphorylation cascades that alter the density of AMPA-type glutamate receptors at excitatory synapses; however, the crucial phosphorylated substrates remain uncertain. Here, we show that the AMPA receptor-associated protein stargazin is quantitatively phosphorylated and that stargazin phosphorylation
Aichurok Kamalova et al.
Cell reports, 32(3), 107921-107921 (2020-07-23)
The anterior thalamus (AT) is critical for memory formation, processing navigational information, and seizure initiation. However, the molecular mechanisms that regulate synaptic function of AT neurons remain largely unexplored. We report that AMPA receptor auxiliary subunit GSG1L controls short-term plasticity
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