AB9220

Anti-Nanog Antibody

serum, Chemicon^®

• Sinónimos: Anti-NANOG/STM1, Anti-STM1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, human
• fabricante / nombre comercial: Chemicon^®
• técnicas: flow cytometry: suitable; western blot: suitable
• entrada: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• Nº de acceso NCBI: NM_024865.2
• Nº de acceso UniProt: Q9H9S0
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... NANOG(79923) ; mouse ... Nanog(71950)

Descripción

Descripción general

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

Especificidad

Recognizes Nanog, human.

Inmunógeno

Synthetic peptide from human Nanog.

Aplicación

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.

Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.

Optimal working dilutions must be determined by the end user.

Descripción de destino

39 kDa (MW 34 kDa)

Forma física

Rabbit Serum. Liquid.

Unpurified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Whole cell lysate from mouse embryonic stem cells

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable