AB6000

Anti-TIMP-3 Antibody, CT

from rabbit, purified by affinity chromatography

• Sinónimos: MIG-5, TIMP metallopeptidase inhibitor 3, Tissue inhibitor of metalloproteinases 3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: horse, human, rat
• reactividad de especies (predicha por homología): pig (based on 100% sequence homology), primate (based on 100% sequence homology), equine (based on 100% sequence homology), canine (based on 100% sequence homology), monkey (based on 100% sequence homology), mouse (95% homology), bovine (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_000353
• Nº de acceso UniProt: P35625
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TIMP3(7078)

Descripción

Descripción general

Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix.

Especificidad

The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2.

Inmunógeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus.

Aplicación

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.

Calidad

Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.

Descripción de destino

Bands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent.

Ligadura / enlace

Replaces: AB802

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HL-60 cell lysate in PMA-conditioned media.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable